Effect of 0.005M CaCl-2 and 0.14M NaCl on the yield and extractibility of histones from rat liver nuclei
Ganelina, L.S.; Ovchinnikova, L.P.; Ianushka, A.L.
Tsitologiia 17(7): 808-813
1975
ISSN/ISBN: 0041-3771 PMID: 1162745 Document Number: 88401
The release and extraction of histones from the nuclei of rat liver under the influence of 0.005 M CaCl2 and 0.14 M NaCl was investigated. A considerable loss of nuclear histones, especially lysine-rich ones, was shown biochemically and cytophotometrically. The preliminary incubation of the nuclei in the respective salt solutions resulted in a more complete extraction of histones. This may be explained by a weaker binding capacity of histones to DNA under the latter conditions. Electrophoresis in polyacrylamid gel showed an increased mobility of histone fractions removed from the nuclei by incubation in the salt solutions.