Top Abstract Purchase Related
Home
>
Node
>
Subnode

Demonstration of membrane-bound immunoglobulin determinants and the significance of a quantitative characterization of the antisera used

Emmrich, F.; Storch, W.; Von Baehr, R.; Schwenke, H.

Folia Biologica 21(3): 155-159

1975

ISSN/ISBN: 0015-5500
PMID: 51802
Document Number: 88093
Lymphocytes from 22 healthy blood donors were tested for the presence of immunoglobulin determinants on their surfaces by indirect membrane immunofluorescence. The proportion of MemIF+ cells was, on the average, 8.7% (3-18) for IgM and 15.8% (8-28) for IgG. The small differences in the proportion of MemIF+ lymphocytes upon use of various anti-IgM sera could be attributed mainly to the different antibody concentrations. The importance of a quantitative characterization of the antisera used was pointed out.

Document emailed within 1 workday
Secure & encrypted payments

Fiebig, H.; Scherbaum, I.; Ambrosius, H. 1980: Evolutionary origin of the T-lymphocyte receptor. II. Production and partial characterization of antisera to the immunoglobulin-like cell membrane protein from thymocytes of the carp Acta Biologica et Medica Germanica 39(7): 845-853
Rivat, C.; Rivat, L.; Ropartz, C. 1970: Demonstration of antigenic determinants bound to the molecular conformation in human immunoglobulins gamma G Progress in Immunobiological Standardization 4: 38-43
Anderson, J.K.; Metzgar, R.S. 1978: Detection and partial characterization of human T and B lymphocyte membrane antigens with antisera to HSB and SB cell lines Journal of Immunology 120(1): 262-271
Tsuji, A.; Torres-Rosado, A.; Arai, T.; Chou, S.H.; Kurachi, K. 1991: Characterization of hepsin, a membrane bound protease Biomedica Biochimica Acta 50(4-6): 791-793
Erdmann, E. 1978: Quantitative aspects of the specific binding of cardiac glycosides to membrane-bound receptors Fortschritte der Medizin 96(35): 1758
Schonwetter, B.S.; Luedtke, R.R.; Kung, M.P.; Billings, J.; Kung, H.F.; Molinoff, P.B. 1989: Characterization of membrane-bound and soluble D2 receptors in canine caudate using [125I]IBZM Journal of Pharmacology and Experimental Therapeutics 250(1): 110-116
Stein, G.; Stelzner, A. 1979: The clinical value of unspecific humoral immune parameters. Attempt at an immunogram. 1. Quantitative determination of IgG, IgM, IgA and IgD and demonstration of antibodies against cell and tissue bound antigens in patients with chronic pyelonephritis Zeitschrift für die Gesamte Innere Medizin und ihre Grenzgebiete 34(4): 117-123
Tsutsumi, Y. 1984: Relation between frequency of the development of enzyme-bound immunoglobulin and pathophysiology. LDH-binding immunoglobulin-- estimation of its frequency in normal subjects Rinsho Byori. Japanese Journal of Clinical Pathology Spec no 60: 85-89
Rodkey, L.S.; Conrad, A.H. 1972: Interspecies occurrence of immunoglobulin antigens reactive with anti-allotype antisera Journal of Immunology 109(2): 342-347
Manning, D.D.; Jutila, J.W. 1974: Prevention of myeloma growth in BALB-c mice by injection of anti-immunoglobulin antisera Journal of Immunology 113(1): 78-83
Burlakova, E.B.; Kaĭrane, C.B.; Molochkina, E.M.; Khokhlov, A.P. 1984: Changes in external membrane lipids of the mouse liver mitochondria and kinetic parameters of membrane-bound monoaminoxidase in vivo and in vitro Voprosy Meditsinskoi Khimii 30(1): 66-72
German, G.P. 1985: Isolation of antisera to different IgA determinants and an analysis of the antigenic structure of human monoclonal IgA Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 6: 52-58
Allegrezza-Giulietti, A.; Serretti, R.; Beccerica, E.; Ferretti, G.; Cervini, C. 1991: Beta-thromboglobulin and polymorphonuclear leukocytes activation. (Effects on chemiluminescence, release of membrane bound calcium, NADPH-oxidase activity and membrane fluidity) Biochemistry International 24(2): 273-279
Morell, A.; Skvaril, F.; Barandun, S. 1973: Qualitative and quantitative investigations on the reactions of normal and myeloma IgG with antisera to IgG Clinical and Experimental Immunology 13(2): 293-302
Esterbauer, H.; Nöhammer, G.; Schauenstein, E.; Weber, P. 1973: Quantitative histochemical demonstration of sulfhydrylgroups by the DDD-staining-method. 3. Quantitative cytospectrometrical determination in Ehrlich-ascites-tumour cells Acta Histochemica 47(1): 106-114
Billing, R.; Terasaki, P.I. 1974: Human leukemia antigen. I. Production and characterization of antisera Journal of the National Cancer Institute 53(6): 1635-1638
Alejandre, M.J.; Garcia-Gonzalez, M.; Segovia, J.L. 1988: Correlation between changes in membrane lipid composition induced by dietary lipid and membrane-bound enzyme activity in chick liver Biochemistry International 17(3): 461-469
Kuśnierczyk, P.; Wysocka, M.; Szkudlarek, J.; Steuden, I.; Szymaniec, S.; Kisielow, P. 1986: Quantitative analysis of tissue distribution of Ly10-like murine alloantigen(s) with antisera and monoclonal antibodies Archivum Immunologiae et Therapiae Experimentalis 34(4): 409-419
Aihara, Y.; Tadokoro, I.; Katoh, K.; Minami, M.; Okuda, K. 1983: T cell allotypic determinants encoded by genes linked to the immunoglobulin heavy chain locus. I. Establishment of monoclonal antibodies against allotypic determinants Journal of Immunology 130(6): 2920-2925
McCoy, J.P.; Hofheinz, D.E.; Ng, A.B.; Nordqvist, S.; Haines, H.G. 1979: Tumor-bound immunoglobulin in human gynecologic cancers Journal of the National Cancer Institute 63(2): 279-283