Evolutionary origin of the T-lymphocyte receptor. II. Production and partial characterization of antisera to the immunoglobulin-like cell membrane protein from thymocytes of the carp
Fiebig, H.; Scherbaum, I.; Ambrosius, H.
Acta Biologica et Medica Germanica 39(7): 845-853
1980
ISSN/ISBN: 0001-5318 PMID: 6163269 Document Number: 157645
An Ig-like protein from Triton N-100 solubilized cell surface proteins from thymocytes can be isolated by specific precipitation using anti-IgM antibodies. Immunizing rabbits with those immune complexes elicited anti-thymocyte mIg sera reacting with both thymocyte mIg and IgM. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that anti-IgM and anti-thymocyte mIg sera recognize the same thymocytic surface protein. The cross-reactivity between thymocyte mIg and IgM is caused by protein determinants very similar to each other. Anti-thymocyte mIg sera absorbed with IgM lose their capacity to bind thymocyte mIg. Following absorption with thymocytes anti-IgM sera still possess amounts of antibodies reacting with humoral IgM.