5-Hydroxytryptamine-14C and dexamphetamine-14C uptake by fundal sarcolemma preparations: effect of thermal and chemical alterations as indirect approach to the problem of the common receptor
Angelis, L.; Predominato, M.; Vertua, R.
Archives Internationales de Pharmacodynamie et de Therapie 216(1): 51-56
1975
ISSN/ISBN: 0003-9780 PMID: 1164105 Document Number: 84973
By submitting a sarcolemma preparation from gastric fundus to thermal (47 degrees C) and chemical (2 M urea) alterations, a significant decrease of the binding of labelled serotonin and dexamphetamine, 40% and 30% respectively, is observed. This finding suggests that the receptors for these two drugs contain a protein moiety, which undergoes a structural and functional modification upon the thermal or chemical procedure employed. The strictly similar behaviour of the binding properties of serotonin and dexamphetamine, in our experimental conditions, gives a strong indirect support to the hypothesis of a common receptor for these two drugs.