Detection of the causative agent of tularemia in the organs of animals in the early stages of development of the infection
Olsuf'ev, N.G.; Ananova, E.V.; Meshcheriakova, I.S.; Savel'eva, R.A.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 10: 13-17
1975
ISSN/ISBN: 0372-9311 PMID: 1108514 Document Number: 83361
A study was made of a possibility of detection of the causative agent of tularemia in the organism of albino mice at early stages of development of the infection after subcutaneous infection with 1, 10 and 100 microbial cells of strains No. 503/834 (Holarctic race) and Schu (nearctic race). The following examinations were made: cultivation on nutrient media, immunofluorescent study, the antibody neutralization test and the passive hemagglutination test with erythrocytic diagnostic agents. The microbe could be regularly revealed three days after the infection. Detection of the causative agent was possible in individual cases at the earlier dates by seeding and by the fluorescent antibody method. Although by cultivation it is possible to reveal individual microbes, but the growth appears on the 3rd--5th day and later. The most rapid response (1.5--2 hours) results (on the presence of the microbe) can be obtained with the aid of the fluorescent antibody method. Application of the mentioned tests with the erythrocytic diagnostic agents permits to obtain data not only on the presence of the causative agent, but also on its quantity of the causative agent in the organism of the infected animal. The mentioned methods provide the most complete characteristics of the dynamics of the accumulation of the microbe in the animal organism.