Pantothenic acid metabolism in chickens during early ontogenesis
Utno, L.I.
Prikladnaia Biokhimiia i Mikrobiologiia 10(5): 691-696
1974
ISSN/ISBN: 0555-1099 PMID: 4463359 Document Number: 77905
Plymouth Rock chicks from hatching were fed a diet based on maize 77, casein 16, gelatin 2, CaCO3 1, NaCl 0.5% and fish oil, without or with D-pantothenic acid (PA) 5, 10 or 20 mg/kg diet. In another trial White Leghorn chicks were given a diet of casein 30, starch 30, glucose 21 and maize oil 10% without or with PA 15 mg/kg. At intervals free and bound PA were estimated in liver by the growth-promoting effect on Saccharomycodes ludwigii and in muscle by the method of Kaplan and Lipmann (NAR 18, 1921).Changes in liver PA were biphasic; during the first 7 to 10 days from hatching bound PA fell significantly while free PA rose slightly, whereas from 10 days the free form rose quickly and the bound form no longer decreased or rose slightly. The second phase occurred only with additional PA 10, 15 or 20 mg/kg. In chicks deprived of PA free and bound PA in liver were 1/10 and 1/3 of those of chicks on the diet with PA 15 mg/kg. Increasing the PA content in the diet reduced the rate of binding of PA in muscle. With the deficient diet muscle PA fell as growth progressed; bound PA in muscle was proportional to that in liver only when the diet was deficient in PA. There was a positive correlation between free PA in liver and the rate of synthesis of muscular proteins.