The use of mosquitoes to detect and propagate dengue viruses
Rosen, L.; Gubler, D.
American Journal of Tropical Medicine and Hygiene 23(6): 1153-1160
1974
ISSN/ISBN: 0002-9637 PMID: 4429185 Document Number: 73668
Parenteral inoculation of Aedes albopictus (Skuse) was found to be a much more sensitive method to detect dengue viruses than was plaque assay in LLC-MK2 cells, which was the most sensitive dengue isolation system known heretofore. This was true whether the viruses were present in sera from naturally infected men or had been 'adapted' to LLC-MK2 cells or newborn mice. Males of A. albopictus were as susceptible to infection as females and could be used without the safety precautions necessary for the latter. All four types of dengue virus replicated to high titre in both male and female mosquitos and high titred pools of virus could be prepared from these insects without the possible modifying effects of 'adaptation' to cell cultures or mice.