Interaction between long noncoding RNA and environmental factors on coronary heart disease
Sun, Y.; Lin, J.; Huang, Q.; Fan, L.; Lin, S.; Li, H.; Wu, S.
Wei Sheng Yan Jiu 48(3): 358-365
2019
ISSN/ISBN: 1000-8020 PMID: 31133116 Document Number: 698433
To investigate the role of leukocyte long noncoding RNA(lncRNA) and environmental factors on coronary heart disease(CHD). A case-control study was conducted in a hospital in Fuzhou City by using stratified random sampling method during March 2015 and December 2017. The related surveys included questionnaire investigation, physical examination and laboratory detection. And real-time fluorescence quantitative PCR was used to detect the expression of lncRNA(NR_027032, NR_047116 and NR_104181) in case group and control group. Multivariate Logistic regression models and crossover analysis were used to analyze the interaction and association of environmental factors and lncRNA on CHD. Wilcoxon rank sum test indicated that: The expression levels of NR_027032, NR_047116 and NR_104181 in CHD were lower than those in control group, the difference was statistically significant(P<0.05). Multivariate Logistic regression analysis showed that the low expression of NR_027032, NR_047116 and NR_104181 may increase the risk of CHD. Crossover analysis showed that the risk of CHD was 19.053 times(95% CI 5.159-70.361) in the presence of overweight and low expression of NR_047116 than that in the normal condition. And the additive model between the two groups was statistically significant(U=1.999, P=0.046). The evaluation indexes were S=0.598, AP=10.070 and RERI=0.629. CHD is the result of environmental factors and genetic factors. The association and additive effect of NR_047116 low expression and overweight may increase the risk of CHD.