Mechanisms of stem cell-derived extracellular vesicles protecting Leydig cells from acute oxidative injury in rats
Zhong, L.; Ju, G.-Q.; Liu, G.-H.; Sun, J.
Zhonghua Nan Ke Xue 24(1): 14-18
2018
ISSN/ISBN: 1009-3591 PMID: 30157354 Document Number: 696605
目的： 揭示干细胞微囊改善Leydig细胞急性氧化应激损伤的机制。方法： 实验组采用单侧睾丸切除的SD大鼠5例建立睾丸缺血-再灌注损伤模型，经尾静脉注射干细胞微囊进行干预。对照组采用单侧睾丸切除的SD大鼠5例经尾静脉注射等量PBS。再灌注2 h后HE染色检测睾丸组织中中性粒细胞浸润程度，TUNEL和caspase 3免疫组化染色检测Leydig细胞凋亡，Ki67免疫组化染色检测Leydig细胞增殖。结果： 实验组大鼠睾丸间质血管中中性粒细胞黏附明显少于对照组，且TUNEL及caspase 3免疫组化结果显示Leydig细胞的凋亡数量明显少于对照组，Ki67免疫组化结果显示实验组Leydig细胞增殖数量多于对照组。结论： 干细胞微囊可减少中性粒细胞在急性期的黏附，通过减少活性氧自由基的来源改善Leydig细胞的急性氧化应激损伤。.
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