Development of tri-component antibacterial hybrid fibres for potential use in wound care
Masood, R.; Hussain, T.; Miraftab, M.; Ali Raza, Z.; Ullah, A.; Areeb, T.; Umar, M.; Riaz, R.
Journal of Wound Care 27(6): 394-402
2018
ISSN/ISBN: 0969-0700 PMID: 29883286 Document Number: 696606
Following on from the debate in the House of Lords (London, UK) 'Improving the standard of wound care in the NHS' in November 2017, Rt Hon. Lord Hunt of Kings Heath set up a meeting on 22 May to discuss the further action to be taken and work that has started to establish a National Wound Care Strategy. Tri-component antibacterial psyllium-alginate-chitosan fibres were developed and their properties were studied with reference to their application in health-care. Psyllium was co-extruded with sodium alginate as a carrier into a coagulation bath containing calcium chloride and hydrolysed chitosan. Different concentrations of the hydrolysed chitosan were used and an in vitro assessment of antibacterial activity of the produced fibres was carried out against the known pathogens of Staphylococcus aureus and Escherichia coli. The effect of hydrolysed chitosan bath composition on physical and mechanical properties of produced fibres was also examined. Chitosan-containing fibres demonstrated a 70-130% thicker dry diameter than the control fibre (F1). The linear density of the fibre increased from 6.8 to 10 tex as the chitosan concentration increased from 10g/l to 30g/l (fibre type F1 to F4). With the addition of hydrolysed chitosan, distilled water absorption was increased while the saline and solution-A (0.83% w/v NaCl and 0.03% w/v CaCl2) absorption decreased. The percentage strain of hybrid fibres was lower than the control fibre due to the inclusion of hydrolysed chitosan. At lower viscosities of the hydrolysed chitosan bath, the fibres were much stiffer due to better penetration of the hydrolysed chitosan. Similarly, at lower viscosities, the tenacities of the hybrid fibres were higher than the control fibre. The hydrolysed chitosan-treated fibres were more effective against Staphylococcus aureus than the Escherichia coli, and the antibacterial activity increased with the decrease in viscosity of the hydrolysed chitosan bath. We developed novel PAC fibres. Antibacterial testing showed that hydrolysed chitosan was more effective against Gram-positive bacteria than Gram-negative bacteria.