ANALYSIS OF EFFECTIVENESS OF USINGJIgY FROM CHICKEN IN SANDWICH METHOD OF HBsAg TESTING
Pechelyulko, A.A.; Tarakanova, Y.N.; Dmitriev, A.D.; Massino, Y.S.; Segal, O.L.; Lavrov, V.F.; Dmitriev, D.A.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 3: 44-51
2016
ISSN/ISBN: 0372-9311 PMID: 30695452 Document Number: 689414
Study antigen-binding ability of polyclonal antibodies (PCA) of chicken compared with monoclonal -antibodies (MCA) of mice in the model of interaction with HBsAg. Mice MCA 18C8 and MKA F3/F4 (IgG) were used, effective in enzyme immunoassay sandwich method of HBsAg determination (with a minimal detection dose of 0.017 ng/ml), and affinity purified anti-HBsAg PCA of chicken (IgY), obtained from 2 immunized birds (PCA No. 1 and PCA No. 2). The ability of antibodies to bind HBsAg was evaluated by analytical sensitivity (slope of binding curve) of solid-phase enzyme immunoassay system using mice MCA and chicken PCA. PCA No. 2 has provided a statistically significant 40% increase of analytical sensitivity, compared with <