Cytotoxic effect of linear alkylbenzene sulfonate on human Chang liver cells
Zhao, W.; Zhao, H.; Wang, K.; Zhang, W.; Wang, J.
Wei Sheng Yan Jiu 45(2): 263-267
2016
ISSN/ISBN: 1000-8020 PMID: 27301227 Document Number: 687128
To investigate the toxicity of linear alkylbenzene sulfonate (LAS) on human Chang liver cells. Chang liver cells were cultured in complete growth medium, RPMI 1640 supplemented with 10% fetal calf serum and divided into control group (cultured with normal medium without LAS), LAS low, medium, high groups (37.57, 75.0 and 150.0 mg/L). After being incubated for 24 h, morphological changes were observed under inverted microscope. The proliferation rate was measured by MTT assay. The injury of cells was evaluated through levels of malondialdehyde (MDA) lactate dehydrogenase (LDH) and aspartate amine transfer (AST). Apoptosis and necrosis of Chang liver cells were explored using Hoechst 33342 and PI staining. When Chang liver cells were treated with different concentrations of LAS, morphological changes of cells were shown that there were black grains in the surface, the number of cells decreased, cast-off cells and cell breakage increased and there were large numbers of necrotic cells in higher-dose groups. Compared with the control group, the cell proliferation was inhibited, the levels of MDA, LDH and GOT increased and the rates of apoptosis and necrosis enhanced significantly because of the treatment of LAS. LAS can injure Chang liver cells in a way, it can inhibit cell proliferation and induce cells apoptosis.