Top Abstract Purchase Related
Home
>
Node
>
Subnode

The Influence of Rifampicin Resistant Mutations on the Biosynthesis of Exopolysaccharides by Strain Escherichia coli K-12 lon

Hovhannisyan, H.G.; Barseghyan, A.H.

Prikladnaia Biokhimiia i Mikrobiologiia 51(5): 490-494

2015

ISSN/ISBN: 0555-1099
PMID: 26596085
Document Number: 681964
The influence of RNA polymerase (rif) mutations on the yield of capsular exopolysaccharide--colanic acid (CA) of Escherichia coli K-12 lon strain was studied. Five colanic acid isogenic producing strains were created by transduction transfer of rif alleles possessing pleiotropic effects. The obtained isogenic strains differed by specific growth rate, size and mucoidness of colonies, the dependence of growth on the medium composition and cultivation temperature, as well as by the adsorption rate of virulent bacteriophage M59, specifically lysing E. coli cells producing CA. Direct correlation between the yield of exopolysaccharides, growth rate and adsorption of bacteriophage M59 was revealed. Among rif recombinants strain AH203, which synthesized twice as much CA compared with the parental strain in submerged cultivation was selected.

Document emailed within 1 workday
Secure & encrypted payments

Mindlin, S.Z.; Danilevskaia, O.N.; Vel'kov, V.V.; Sokolova, E.V. 1973: Genetic study of mutations changing the dominance of alleles of sensitivity of Escherichia coli K-12 RNA polymerase to rifampicin and streptolydigin Genetika 9(12): 84-94
Yoshida, S.; Suzuki, K.; Tsuyuguchi, K.; Iwamoto, T.; Tomita, M.; Okada, M.; Sakatani, M. 2006: Detection of rpoB mutations in rifampicin-resistant Mycobacterium kansasii Kekkaku: 81(7): 475-479
Generozov, E.V.; Al'tshuler, M.L.; Govorun, V.M.; Lopukhin, I.M.; Golyshevskaia, V.I.; Chernousova, L.N.; Khomenko, A.G. 1999: Detection and characteristics of rpoB gene mutations in rifampicin-resistant clinical strains of M. tuberculosis Problemy Tuberkuleza 2: 39-42
González, N.; Torres, M.J.; Palomares, J.C.; Aznar, J. 1998: Characterization of the rpoB gene mutations in clinical isolates of rifampicin-resistant Mycobacterium tuberculosis Enfermedades Infecciosas y Microbiologia Clinica 16(9): 404-407
Gorlenko AhM; Afanas'ev, V.M.; Iarulin, V.R. 1979: Effect of rifampicin and RNA polymerase changing mutation on the spectrum of proteins synthesized in Escherichia coli cells Molekuliarnaia Biologiia 13(6): 1350-1359
Zlotnikov, K.M.; Khmel'nitskiĭ, M.I. 1974: Mutations influencing intragenic recombination in Escherichia coli K-12 Genetika 10(11): 71-76
Zlotnikov, K.M.; Khmel'nitskiĭ, M.I. 1974: Mutations influencing intragenic recombination in Escherichia coli K-12 Genetika 10(11): 71-76
Bandrin, S.V.; Rabinovich, P.M.; Stepanov, A.I. 1983: 3 linkage groups of the genes of riboflavin biosynthesis in Escherichia coli Genetika 19(9): 1419-1425
Snyder, L.; Jorissen, L. 1988: Escherichia coli mutations that prevent the action of the T4 unf/alc protein map in an RNA polymerase gene Genetics 118(2): 173-180
Aĭrapetian, S.N.; Stepanian, R.S.; Oganesian, G.G.; Barsegian, A.A.; Alaverdian, Z.R.; Arakelian, A.G.; Markosian, L.S. 2001: Effect of mechanical stress on lon mutant strain of Escherichia coli K-12 Mikrobiologiia 70(2): 248-252
Pankova, S.V.; Abilev, S.K. 1993: Molecular cloning of threonine biosynthesis genes from Propionibacterium shermanii in Escherichia coli cells Genetika 29(3): 539-542
Androsov, V.V.; Demkin, V.V.; Levashev, V.S. 1977: Morphologic mutant of Escherichia coli K12 having disorders in morphology, division and cell wall biosynthesis Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1977(4): 53-59
Tamari, M.; Horiguchi, M. 1976: Degradation of ciliatine (2-aminoethylphosphonic acid) by the mutant strain Tm-1 of Escherichia coli Seikagaku. Journal of Japanese Biochemical Society 48(8): 810-813
Cherniakova, V.I.; Smirnova, T.V. 1982: Effect of digestive secretions and bile-containing preparations on Escherichia coli strain M-17 Vrachebnoe Delo 5: 97-99
Terzolo, H.R.; Zoratti de Verona, A.; d'Empaire, M.; Furowicz, A.J. 1977: An avian strain of Escherichia coli with antigens common to the genus Salmonella Revista de la Asociacion Argentina de Microbiologia 9(1): 4-10
Bĕlohlávek, S. 1970: Sevacol--a lyophilized bacterial strain of Escherichia coli. I. Production of the drug Ceskoslovenska Farmacie 19(2): 65-66
Alós, J.I.; Rodríguez, C.; Amor, E.; Gómez Garcés, J.L. 1991: Escherichia coli bacteremia resistant to ciprofloxacin Medicina Clinica 97(14): 556-557
Mironov, V.N.; Gordeev, V.K. 1982: Method of selecting mutations for the plasmid genes of streptomycin resistance in Escherichia coli K-12 cells Genetika 18(8): 1289-1293
Wu, Q.; Zhang, J.; Zhao, C.; Zhu, J. 2008: Cloning and sequencing of the papA gene from uropathogenic Escherichia coli 4030 strain Wei Sheng Yan Jiu 37(5): 574-577
Beskrovnaia, O.I.; Fonshteĭn, M.I.; Kolibaba, L.G.; Iankovskiĭ, N.K.; Debabov, V.G. 1988: Molecular cloning and the expression of the genes of amino acid biosynthesis of Corynebacterium glutamicum in Escherichia coli cells Genetika 24(7): 1153-1158