Effect of the dose-fractionation on the frequency of chromosome aberrations induced in mice by TEPA
Srám, R.J.; Zudová, Z.
Folia Biologica 21(1): 58-67
1973
ISSN/ISBN: 0015-5497 PMID: 4120893 Document Number: 67958
The effect of dose fractionation of the chemical mutagen, TEPA (tris-1-aziridinyl-phosphine oxide), on the frequency of chromosome aberrations in mice was studied using the dominant lethal test and cytological analysis of chromosome rearrangements in spermatocytes. ICR male mice were treated intraperitoneally by a single dose or repeated doses of TEPA, the total dose for each animal being 1 mg. TEPA was injected in 2 doses of 0.5 mg at intervals of 4, 12 or 24 h, 5 doses of 0.2 mg at intervals of 12 or 24 h or 10 doses of 0.1 mg spaced 24 h apart. The fractionated doses of TEPA decreased the frequency of dominant lethals compared with the frequency after a single 1-mg dose in the postmeiotic stage of spermatogenesis. A different spectrum of genetic injury was found according to the scheme of fractionation. The cytological analysis of spermatocytes treated at the spermatogonial stage indicated an increased frequency of chromosome rearrangements after fractionated doses of TEPA, but the difference was not statistically significant.