Comparative analysis the frequency and spectrum of chromosome aberrations in irradiated in vitro peripheral blood lymphocytes of the elderly and centenarians from Kyiv
Talan, O.O.; Shemetun, O.V.; Demchenko, O.M.; Papuga, M.S.; Pilinska, M.A.
Problemy Radiatsiinoi Medytsyny Ta Radiobiolohii 22: 231-237
2017
ISSN/ISBN: 2304-8336 PMID: 29286510 Document Number: 694654
Meta. Vstanovyty i porivniaty chastotu aberatsiy̆ khromosom pry diï rentgenivs'kogo vyprominiuvannia in vitro v dozi 0,25 Gr na limfotsyty peryferychnoï krovi osib litn'ogo viku ta dovgozhyteliv.Materialy i metody. Materialom tsytogenetychnogo doslidzhennia sluguvaly limfotsyty peryferychnoï krovi 11 osib litn'ogo viku i 10 dovgozhyteliv, shcho buly opromineni in vitro v dozi 0,25 Gr ta kul'tyvuvalys' za zagal'nopryy̆ niatym napivmikrometodom. Preparaty metafaznykh khromosom farbuvaly z vykorystanniam GTG zabarvlennia ta analizuvaly pid mikroskopamy zi zbil'shenniam x 1000.Rezul'taty. Seredn'ogrupovi chastoty aberatsiy̆ khromosom pry oprominenni krovi in vitro perevyshchuvaly taki bez oprominennia (r < 0,001) i stanovyly 11,60 ± 0,95 ta 6,82 ± 0,63 na 100 klityn u osib litn'ogo viku i dovgozhyteliv, vidpovidno. Radiatsiy̆no indukovane zrostannia chastoty poshkodzhen' khromosom vidbuvalos' za rakhunok aberatsiy̆ khromosomnogo typu, shcho ie markeramy diï ionizuiuchogo vyprominiuvannia. U osib litn'ogo viku zareiestrovano pidvy shchenu chastotu aberatsiy̆ khromatydnogo typu, shcho vvazhaiet'sia oznakoiu khromosomnoï nestabil'nosti.Vysnovky. Otrymani rezul'taty svidchat' pro pidvyshchenu chutlyvist' limfotsytiv krovi liudyny u litn'omu vitsi do oprominennia v maliy̆ dozi ta dozvoliaiut' prypustyty perevagu osib z genetychno determinovanoiu khromosomnoiu stabil'nistiu u dosiagnenni dovgolittia.