Modulation of GABA- and kainate-activated currents by metabotropic receptors in isolated rat cortical neurons
Amakhin, D.V.; Popov, V.A.; Veselkin, N.P.
Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova 100(10): 1169-1179
2014
ISSN/ISBN: 0869-8139 PMID: 25697024 Document Number: 677041
Whole-cell patch-clamp recordings from isolated neurons from rat prefrontal cortex have been made to study GABAb and mGluR receptor modulation of currents induced by applications of GABA and kainate. The GABAb-receptor antagonist CGP-55845 (5 microM) enhanced the peak by 26 +/- 13% (n = 6) but had no effect on the steady-state of GABA-activated current. Bath application of GABAb-receptor agonist baclofen (50 microM) enhanced the GABAa currents by 9 +/- 2% (n = 8). Kainate-activated currents were not affected by baclofen. Both GABA-activated currents and kainate-activated currents were not affected by trans-ACPD (MGluR agonist). These results suggest that in cortex postsynaptic response of GABAa-receptors can be modulated by GABAb-receptors.