Effects of different processing methods on effective components and sulfur dioxide residue in Gastrodiae Rhizoma
Ning, Z.-W.; Mao, C.-Q.; Lu, T.-L.; Ji, D.; Liu, J.; Ji, L.; Yang, H.; Wang, F.-Q.
Zhongguo Zhong Yao Za Zhi 39(15): 2814-2818
2014
ISSN/ISBN: 1001-5302 PMID: 25423814 Document Number: 675409
The contents of adenosine, gastrodin, 4-hydroxybenzyl alcohol, 4-hydroxybenzaldehyde, parishin and sulfur dioxide residue were compared in differently-processed Gastrodiae Rhizoma to provide the basis for a reasonable processing method of Gastrodiae Rhizoma. The analysis was performed on a Merck Purospher STAR column (4.6 mm x 250 mm, 5 μm) with a mobile phase consisting of methanol and water (containing 0.1% formic acid) under gradient elution at a flow rate of 1.0 mL x min(-1). The eluates were detected at 270 nm, and the column temperature was 35°C. The content of adenosin, gastrodin, 4-hydroxybenzyl alcohol, 4-hydroxy-benzaldehyde and parishin in processing of boiling or sulfur-fumigated were lower than that of in processing of steaming. Furthermore, the sulfur dioxide residue of sulphur-fumigated groups exceed 400 mg x kg(-1). This stable and reliable method will contribute to the quality control of different processed Gastrodiae Rhizoma.