Optimization of parameters for phenol degradation using immobilized Candida tropicalis SSK01 in batch reactor
Sivasubramanian, S.; Namasivayam, S.K.R.
Journal of Environmental Biology 35(3): 531-536
2014
ISSN/ISBN: 0254-8704 PMID: 24813009 Document Number: 675203
Bioremediation of phenol was done using Candida tropicalis SSK01 immobilized cells isolated from petroleum contaminated soil. Optimization of phenol degradation studies was carried at 30 °C to 40 °C, pH 6 to 8 and initial concentration of 300 mgl⁻¹ to 900 mgl⁻¹. Candida tropicalis SSK01 cells immobilized using sodium alginate were used in phenol degradation studies. Optimization of phenol degradation was performed by Central Composite Design (CCD). A total of 20 experiments were carried out and the optimal degradation of 95.2% was observed at 34.20 °C at pH 6.86 with initial concentration of 610 mgl⁻¹ The R², adjusted R² and Predicted R² values were 0.9976, 0.9955 and 0.9919 respectively which indicates that experimental values are in good agreement with the predicted values.