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The effectiveness of molecular markers for the identification of Lr28, Lr35, and Lr47 genes in common wheat

Gul'tiaeva, E.I.; Orina, A.S.; Gannibal, F.B.; Mitrofanova, O.P.; Odintsova, I.G.; Laĭkova, L.I.

Genetika 50(2): 147-156

2014

ISSN/ISBN: 0016-6758
PMID: 25711022
Document Number: 673182
The effectiveness of molecular markers for the identification of leaf rust resistance genes Lr28, Lr35, Lr47 transferred to common wheat was assessed the using samplesof Triticum spp. and Aegilops spp. from Ae. speltoides. Markers Sr39F2/R3, BCD260F1/35R2 of gene Lr35 and PS10 of Lr47 gene were characterized by high efficiency and were revealed in a line of common wheat containing these genes, and samples of Ae. speltoides (their donor). Marker SCS421 of Lr28gene and markers Sr39#22r, Sr39#50s, BE500705 of Lr35/Sr39 genes turned out to be less specific. Marker SCS421 was amplified in the samples of the T. timopheevii species, and markers Sr39#22r, Sr39#50s--in the Ae. speltoides, Ae. tauschii, T. timopheevii, line KS90WRC010 (Lr41), the sort of common wheat In Memory of Maistrenko, obtained using synthetic hexaploid T. timopheevii x Ae. tauschii and introgressive lines obtained using Ae. speltoides. Marker BE500705, which indicates the absence of Lr35/Sr39 genes, was not revealed in lines TcLr35 and MqSr39, in Ae. speltoides, Ae. tauschii and T. boeoticum (kk-61034, 61038). Analysis of the nucleotide sequences of amplification products obtained with the markers SCS421 and Sr39#22r indicated their low homology with TcLr28 and TcLr35. Using molecular markers, we showed a different distribution of Lr28 (77%), Lr35 (100%) and Lr47 (15%) genes in 13 studied samples ofAe. speltoides. In introgressive lines derived from Ae. speltoides, contemporary Russian sorts of common wheat and triticale variants Lr28, Lr35, Lr47 genes were not revealed.

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Goncharov, N.P.; Efimov, V.M. 2003: Localization of genes, determining quantitative traits in wheat: amendment to the "catalog of chromosomal mapping of genes in domestic cultivars of wheat" Genetika 39(11): 1474-1483
Kozub, N.O.; Sozinov, I.O.; Blium, I.B.; Sozinov, O.O. 2013: Study of the effects of gamma-irradiation of common wheat F1 seeds using gliadins as genetic markers Tsitologiia i Genetika 47(1): 17-25
Khlëstkina, E.K.; Salina, E.A.; Pshenichnikova, T.A.; Arbuzova, V.S.; Koval', S.F. 2000: Analysis of isogenic lines of the soft wheat carrying dominant alleles of Bg, Hg, and Rg1 genes using microsatellite and protein markers Genetika 36(10): 1374-1379
Sabbah, A.; Heulin, M.G.; Drouet, M.; Le Sellin, J.; Bonneau, J.C.; Kalfon, P.; Fourrier, E. 1987: Identification of common antigens of Phleum pollen and wheat. Preliminary study Allergie et Immunologie 19(3): 110-111
Sivolap, I.M.; Topchieva, E.A.; Chebotar', S.V. 2000: Identification and certification of common wheat cultivars using RAPD and SSRP methods of analysis Genetika 36(1): 44-51
Chebotar, G.A.; Chebotar, S.V.; Motsnyy, I.I. 2016: Pleiotropic effects of Gibberellin-Sensitive and Gibberellin-Insensitive Dwarfing Genes in Common Wheat of the Southern Step Region of Black Sea Tsitologiia i Genetika 50(1): 26-35
Grandjouan, S.; Henry, I.; Haliassos, A. 1991: Molecular analysis of genes involved in colorectal tumors. Markers of tumor predisposition and progression Gastroenterologie Clinique et Biologique 15(1): 34-48
Malyshev, S.V.; Dolmatovich, T.V.; Voĭlokov, A.V.; Sosnikhina, S.P.; Tsvetkova, N.V.; Lovtsius, A.V.; Kartel', N.A. 2009: Molecular genetic mapping of the sy1 and sy9 asynaptic genes in rye (Secale cereale L.) using microsatellite and isozyme markers Genetika 45(12): 1634-1640
Hermida, M.; Gibert, I.; Barbé, J.; Roy, C. 1990: Comparative study with digoxigenin and biotin markers for the DNA probe identification of genes coding for type TEM beta-lactamases Enfermedades Infecciosas y Microbiologia Clinica 8(8): 501-504
Kornienko, I.V.; Iakushev, V.V.; Frolova, S.A.; Zemskova, E.I.; Kabanova, N.P.; Ivanov, P.L. 2003: Results of applying molecular-and-genetic markers of chromosomal DNA in identification of unrecognizable remains of servicemen lost in military actions in the Northern Caucasus Sudebno-Meditsinskaia Ekspertiza 46(5): 36-41
Osminin, S.V.; Vetshev, F.P.; Rudenko, V.V.; Zaletaev, D.V.; Khorobrykh, T.V.; Nemtsova, M.V. 2016: The molecular genetic alterations in mucosa of intestines as markers of oncologic progression and estimate of effectiveness of anti-reflux operations in patients with Barrett's esophagus Klinicheskaia Laboratornaia Diagnostika 61(10): 681-685
Van Dongen, J.J. 1988: Immunoglobulin genes and T-cell receptor genes and the expression of immunological markers. I. the construction of the specific immune system Nederlands Tijdschrift Voor Geneeskunde 132(19): 855-862
Hansen, L. 2003: Candidate genes and late-onset type 2 diabetes mellitus. Susceptibility genes or common polymorphisms? Danish Medical Bulletin 50(4): 320-346
Motta, R.; Moutier, R.; Halle-Pannenko, O. 1981: Minor histocompatibility genes important in lethal graft-versus-host reaction (GVHR): chromosomal assignment of five genes using ten chromosomal markers Transplantation Proceedings 13(1 Pt 2): 1207-1214
Klitsov; Artem'eva, G.M.; Lazareva, E.M.; Muromtsev, G.S. 2000: Effect of fusococcin on the genome of common wheat Genetika 36(8): 1071-1080
Faĭt, V.I.; Simonenko, L.K.; Mokanu, N.V.; Popova, N.V. 2007: Chromosomal location of genes for vernalization duration (Vrd) in winter bread wheat Genetika 43(2): 202-208
Cherepakhin, V.V.; Iakubov, L.Z.; Ibragimov, A.R.; Vengerova, T.I.; Rokhlin, O.V. 1986: Analysis of the frequency of allelic exclusion of immunoglobulin light chain genes and molecular characteristics of immunoglobulins secreted by hybridomas with expression of both allelic genes Molekuliarnaia Biologiia 20(6): 1672-1681
Sherban, O.D.; Oliinyk, A.M. 1971: Identification of the wheat stripe mosaic virus in Moldavia Mikrobiolohichnyi Zhurnal 33(1): 56-60
Motsnyĭ, I.I.; Faĭt, V.I.; Blagodarova, E.M. 2009: Identification and characteristics of the 1R (1B) bread wheat substitution strains Tsitologiia i Genetika 43(3): 26-35
Hou, J.-X.; Yang, X.-Q.; Chen, C.; Jiang, Q.; Yang, G.-L.; Li, Y. 2011: Screening the gastric cancer related tumor markers from multi-tumor markers protein chip with kappa coefficient and cost-effectiveness analysis Hepato-Gastroenterology 58(106): 632-636