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Analysis of the frequency of allelic exclusion of immunoglobulin light chain genes and molecular characteristics of immunoglobulins secreted by hybridomas with expression of both allelic genes

Cherepakhin, V.V.; Iakubov, L.Z.; Ibragimov, A.R.; Vengerova, T.I.; Rokhlin, O.V.

Molekuliarnaia Biologiia 20(6): 1672-1681

1986

ISSN/ISBN: 0026-8984
PMID: 3100944
Document Number: 283519
The investigation of 750 B-lymphocyte hybridoma clones obtained by fusion of mouse myeloma and newborn heterozygous Igk-la/Igk-1b rat splenocytes has revealed that 9,8% of Ig kappa-chain genes are rearranged productively. Seventeen hybridomas secrete kappa-chains of both allelic variants. The analysis of IgM molecules of nine such clones demonstrated that in six cases only one L-chain allotype is present in IgM. Thus for the first time the high frequency of selective association of H and L chains was shown. Evidently this selectively may function as one of the allelic exclusion mechanisms at the Ig assembly stage.

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Gustafsson, K.; LeGuern, C.; Hirsch, F.; Germana, S.; Pratt, K.; Sachs, D.H. 1990: Class Ii genes of miniature swine. IV. Characterization and expression of two allelic class Ii DQB cDNA clones Journal of Immunology 145(6): 1946-1951
Buxbaum, J.N.; Chuba, J.V.; Hellman, G.C.; Solomon, A.; Gallo, G.R. 1990: Monoclonal immunoglobulin deposition disease: light chain and light and heavy chain deposition diseases and their relation to light chain amyloidosis. Clinical features, immunopathology, and molecular analysis Annals of Internal Medicine 112(6): 455-464
Alvarez, J.D.; Anderson, S.J.; Loh, D.Y. 1995: V(D)J recombination and allelic exclusion of a TCR beta-chain minilocus occurs in the absence of a functional promoter Journal of Immunology 155(3): 1191-1202
Tatarskyy, P.; Kucherenko, A.; Livshits, L. 2010: Allelic polymorphism of F2, F5 and MTHFR genes in population of Ukraine Tsitologiia i Genetika 44(3): 3-8
Chorna, L.B.; Akopian, H.R.; Makukh, H.V.; Fedoryk, I.M. 2011: Allelic polymorphism of MTHFR, MTR and MTRR genes in patients with cleft lip and/or palate and their mothers Tsitologiia i Genetika 45(3): 51-56
Tsarev, V.N.; Nikolaeva, E.N. 2007: The allelic polymorhism of IL-1alpha and IL-beta genes in patients with chronic inflammatory periodontal diseases Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 2007(3): 43-47
Qiu, X.; Singal, D.P. 1995: Allelic polymorphism in the upstream regulatory region of HLA-DRB genes: functional role of conserved consensus motifs Transplantation Proceedings 27(1): 682-683
Mettimano, M.; Lanni, A.; Migneco, A.; Specchia, M.L.; Romano-Spica, V.; Savi, L. 2001: Angiotensin-related genes involved in essential hypertension: allelic distribution in an Italian population sample Italian Heart Journal: Official Journal of the Italian Federation of Cardiology 2(8): 589-593
Van Dongen, J.J. 1988: Immunoglobulin genes and T-cell receptor genes and the expression of immunological markers. I. the construction of the specific immune system Nederlands Tijdschrift Voor Geneeskunde 132(19): 855-862
Koch, O.M.; Volkenandt, M.; Göker, E.; Buer, J.; Probst, M.; Banerjee, D.; Danenberg, P.V.; Bertino, J.R. 1994: Molecular detection and characterization of clonal cell populations in acute lymphocytic leukemia by analysis of conformational polymorphisms of cRNA molecules of rearranged T-cell-receptor-gamma and immunoglobulin heavy-chain genes Leukemia 8(6): 946-952
Mami, F.; Kindt, T.J. 1987: C lambda 2 and C lambda 4 immunoglobulin light chain genes in a wild-derived inbred mouse strain Journal of Immunology 138(11): 3980-3985
Ghaffari, S.H.; Lobb, C.J. 1993: Structure and genomic organization of immunoglobulin light chain in the channel catfish. An unusual genomic organizational pattern of segmental genes Journal of Immunology 151(12): 6900-6912
Fomicheva, I.I. 1992: Immunogenetics of immunoglobulins in domestic mink. VII. Features of phenotypic expression of gamma-heavy chain constant region allotypes mask linkage of IgCH genes Genetika 28(8): 142-152
Feddersen, R.M.; Martin, D.J.; Van Ness, B.G. 1990: Novel recombinations of the IG kappa-locus that result in allelic exclusion Journal of Immunology 145(2): 745-750
Vincent, R.; Louis, P.; Gongora, C.; Papa, I.; Clot, J.; Eliaou, J.F. 1996: Quantitative analysis of the expression of the HLA-DRB genes at the transcriptional level by competitive polymerase chain reaction Journal of Immunology 156(2): 603-610
Litwin, S.D.; Hütteroth, T.H.; Lin, P.K.; Kennard, J.; Cleve, H. 1974: Immunoglobulin expression of cells from human lymphoblastoid lines. II. Interrelationship among surface, cellular, and secreted immunoglobulins Journal of Immunology 113(2): 668-672
Singal, D.P.; D'Souza, M.; Reid, B.; Kassam, Y.; Buchanan, W.W.; Blajchman, M.A. 1989: Molecular analysis of the HLA-D region and the T-cell antigen receptor beta-chain genes in monozygotic twins discordant for rheumatoid arthritis Transplantation Proceedings 21(1 Part 1): 632-634
Fridman, W.H. 1988: 500 genes, 50 million proteins: Susumu Tonegawa and the rearrangement of immunoglobulin genes Pathologie-Biologie 36(2): 117-119
Korabiowska, M.; Tscherny, M.; Stachura, J.; Ruschenburg, I.; Cordon-Cardo, C.; Brinck, U. 2002: Relationship between DNA mismatch repair genes expression, Ku-genes expression and ploidy-related parameters in the progression of pigmented lesions of the skin In Vivo 16(5): 317-321
Kappes, D.J.; Arnot, D.; Okada, K.; Strominger, J.L. 1984: Structure and polymorphism of the HLA class II SB light chain genes EMBO Journal 3(12): 2985-2993