Synthesis and identification of artificial antigen of forsythin
Ren, Y.-J.; Qu, H.-H.; Cheng, J.-J.; He, N.-N.; Feng, S.-L.; Zhao, L.-L.; Zhao, Y.; Wang, Q.-G.
Zhongguo Zhong Yao Za Zhi 39(12): 2295-2299
2014
ISSN/ISBN: 1001-5302 PMID: 25244762 Document Number: 671984
The establishment of high specificity and sensitivity method of small molecule monoclonal antibody-based immunoassay has a great importance in the study of small molecule compounds in Chinese medicine, wherein synthesis of small molecule artificial antigen is a critical step in the preparation of small molecule antibodies. Oxidation method using sodium iodide was used to synthesize immunogenic antigen (FRn-BSA) and coating antigen (FRn-OVA) of forsythin. UV spectroscopy and thin layer chromatography showed that forsythin was successfully conjugated with BSA and OVA. After immuned FRn-BSA, the mice could specifically produce anti-forsythin antibodies with titer up to 1:8 000, and the linear range was from 1 mg x L(-1) to 100 mg x L(-1). In this paper, the artificial antigen of forsythin was successfully synthesized, which can be applied for preparation of monoclonal antibodies and establishment of appropriate immune method.