Local level of neoangiogenesis factors in dynamics of experimental metastatic process in liver
Frantsiiants, E.M.; Kaplieva, I.V.; Trepitaki, L.K.; Pogorelova, I.A.; Gorina, I.I.
Eksperimental'naia i Klinicheskaia Gastroenterologiia 12: 56-60
2014
ISSN/ISBN: 1682-8658 PMID: 26058113 Document Number: 671570
The aim--to study the dynamics content of VEGF-A, VEGF-R1, EGF and EGF-R1 in liver and spleen tissues from rats at different stages of liver metastases. We have designed a model of liver metastases in male rats. The content of growth factors was examined by ELISA at 1, 2 and 5 weeks of carcinogenesis in tissue of liver and tumors of spleen. The content of growth factors increased in liver tissue at different stages of metastasis. The concentration of VEGF-A increased gradually from the 1st to 5th week carcinogenesis. The concentration of VEGF-R1 increased after 2 weeks and decreased slightly after 5 weeks of carcinogenesis. The level of all components of EGF/EGF-R1 increased after 2 weeks of carcinogenesis. The following pathogenetic aspects of liver metastasis were defined: change in levels of VEGF-A/VEGF-R1 indicating the progressive development of the process of neoangiogenesis, and increased levels of EGF/EGF-R1 responsible for metastasis processes.