Effects of chain length of polyacrylic acid (PAA) on proteins adsorption of polystyrene-polyacrylic acid (PS-PAA) spherical polyelectrolyte brushes
Liu, Y.; Wen, Y.; Xu, H.; Guo, X.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 30(2): 421-427
2013
ISSN/ISBN: 1001-5515 PMID: 23858773 Document Number: 667706
We studied the interaction between proteins and polystyrene-polyacrylic acid (PS-PAA) spherical polyelectrolyte brushes with different polyacrylic acid (PAA) chain lengths, including the physical adsorption and chemical adsorption in PBS buffer. Results showed that the amount of bovine serum albumin (BSA) physically adsorbed on PS-PAA spherical polyelectrolyte brushes decreased to a minimum of 33 microg/mg whereas the amount of streptavidin (SA) chemically adsorbed increased with the increase of chain length and carboxyl quantity. The biotin binding capacity of streptavidin chemically adsorbed on PS-PAA spherical polyelectrolyte brushes was roughly evaluated via enzyme competitive inhibition.