Selective proteolysis of abnormal proteins of shortened chain length in rabbit reticulocytes
Hipkiss, A.R.; McKay, M.J.; Daniels, R.S.; Worthington, V.C.; Atkinson, E.M.
Acta Biologica et Medica Germanica 40(10-11): 1265-1275
1981
ISSN/ISBN: 0001-5318 PMID: 7043992 Document Number: 176530
Proteolysis of certain shortened globin chains can proceed in cell-free extracts of rabbit reticulocytes in the absence of ATP. Proteolysis of amino acid analogue-induced abnormal globin chains of normal chain length may be stimulated by ATP, however. The ATP independent degradation of globin cyanogen bromide-peptides appears to require the presence of free, unblocked amino groups in the substrate, has a pH optimum of 7.8 and is inhibited by phenanthroline, cobalt and zinc ions, N-ethylmaleimide and cystamine.