Analysis of primary metabolites of ranolazine in dog urine by LC-MS (n)
Tang, J.-y.; Wang, W.; Li, L.; Zhang, C.-g.; Wang, D.-m.; Sun, L.
Yao Xue Xue Bao 47(8): 1031-1038
2012
ISSN/ISBN: 0513-4870 PMID: 23162900 Document Number: 663377
Ranolazine and metabolites in dog urine were identified by LC-MS(n). Dog urine samples were collected after ig 30 mg x kg(-1) ranolazine, then the samples were enriched and purified through solid-phase extraction cartridge. The purified samples were analyzed by LC-MS(n). The possible metabolites were discovered by comparing the full scan and SIM chromatograms of the test samples with the corresponding blanks. Seventeen phase I metabolites and fourteen phase II metabolites were identified in dog urine. Three metabolites were identified by comparing with the control article. The metabolites were formed via the following metabolic pathways: O-demethylation, O-dearylation, hydroxylation, N-dealkylation, amide hydrolysis, glucuronidation and sulfation. The LC-MS(n) method is suitable for the rapid identification of drug and its metabolites in biologic samples.