Genotypes 4 and 5 of the tick-borne encephalitis virus: features of the genome structure and possible scenario for its formation
Demina, T.V.; Dzhioev, I.P.; Kozlova, I.V.; Verkhozina, M.M.; Tkachev, S.E.; Doroshchenko, E.K.; Lisak, O.V.; Paramonov, A.I.; Zlobin, V.I.
Voprosy Virusologii 57(4): 13-19
2012
ISSN/ISBN: 0507-4088 PMID: 23012978 Document Number: 660865
On the basis of the comparison of complete genome structures of 32 strains and gene E fragments (160 ndt) of 643 strains and RNA isolates of tick-borne encephalitis (TBE) virus, we confirmed our previously expressed assumption (Zlobin V.I. et al, 2001) of existence, along with the three major genotypes, of genotypes 4 (strain 178-79) and 5 (strain 886-84). "Mosaic" structure of the polyprotein in the two strains was established. It manifests itself in particular in the sequences of 14 positions (C-3, E-206, NS1-54, NS-285, NS2A-100, NS2A-127, NS2A-174, NS2A-175, NS2A-225, NS3-376, NS4B-28, NS4B-96, NS5-18, NS5-671) containing the amino acids strictly conserved for each of the three major genotypes and is consistent with a uniform pattern of distribution of nucleotide substitutions that are specific for genotypes 1, 2 and 3. Possible scenario of the origin of TBE genotypes 4 and 5 was suggested.