Effect of TRA-system of plasmids RP4 and R68.45 on pseudomonas mallei virulence
Verevkin, V.V.; Volozhantsev, N.V.; Miakinina, V.P.; Svetoch, E.A.
Vestnik Rossiiskoi Akademii Meditsinskikh Nauk 6: 37-40
1997
ISSN/ISBN: 0869-6047 PMID: 9289278 Document Number: 6608
In Pseudomonas mallei, spontaneous mutants (Tra- mutants) of the plasmids RP4 and R68.45, losing the ability to transfer at conjugation are formed. The plasmids RP4 and R68.45 with Tra(+)-phenotype caused a decrease in P. mallei virulence for laboratory animals. At the same time, Tra- mutants of these plasmids do not affect P. mallei virulence. The insertion of DNA fragment of about 1900 bp into the plasmid transfer gene regions (tra-2-tra-3) gave rise to RP4 and R68.45 tra mutations detectable on examination.
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