EMS mutation of suspension cells and selection of high temperature tolerant mutants from Pinellia ternata
Tao, X.; Zhang, A.; Xue, J.; Teng, J.; Lu, H.; Xue, T.
Zhongguo Zhong Yao Za Zhi 37(12): 1731-1733
2012
ISSN/ISBN: 1001-5302 PMID: 22997813 Document Number: 659885
To determine the optimal concentration and processing time of EMS mutation for suspension cells from Pinellia ternata. Under four EMS concentration gradients (0.1% , 0.2%, 0.4%, 0.6%) and three processing time gradients (0.5, 1.0, 2.0 h), the suspension cells of P. ternata were mutagenized. The results showed that the survival rate was significantly different under the different concentrations of EMS and the different processing time. In the same processing time, the EMS concentrations were increased, but the suspension cells survival rate decreased gradually. The optimum EMS concentration for the mutagenesis was 0.4% and the best processing time was 1 hour.