Variation of endogenous hormones in formation of microtubers of Pinellia ternata in vitro
Sheng, W.; Xue, J.; Zhang, A.; Chang, L.; Fan, C.
Zhongguo Zhong Yao Za Zhi 35(8): 943-946
2010
ISSN/ISBN: 1001-5302 PMID: 20617668 Document Number: 641543
Through analysis of variation and function of five endogenous hormones (IAA, GA3, ABA, ZR and JA) in the formation of microtubers of Pinellia ternata in vitro to explore the physiological and biochemical mechanism of tubers' development. The endogenous hormones of microtubers were isolated in different stages of tubers expansion when the leaf stalks of P. ternata were cultured on MS + 6-BA 0.5 mg x L(-1) + NAA 0.1 mg x L(-1) + sucrose 5% medium to induce the formation of microtubers, then them and stems were detected with Enzyme-linked Immunosorbent Assays (ELISA). The results showed that the content of IAA, ABA, JA and ZR in the intumescentia period of microtubers were rapidly rising. The curve of GA3 appeared the type of anti-S. IAA, ABA, JA, ZR and GA3 played an important role in controlling formation of microtubers in P. ternata.