Dynamic of myocarditis development in rats after injection of cardiac myosine combined with IFA
Morozova, M.P.; Gavrilova, S.A.; Zemtsova, L.V.; Pogodina, L.S.; Postnikov, A.B.; Chentsov, I.S.
Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova 98(2): 269-282
2012
ISSN/ISBN: 0869-8139 PMID: 22650071 Document Number: 657481
Myocarditis development was investigated after immunization rats with single subcutaneous injection of cardiac myosin (800 microg/kg) with incomplete Freund's adjuvant (IFA) (M + IFA group). Control group received equal volume of IFA alone or nothing (intact group). On days 4, 14, and 21 after injection, light and electron microscopy of heart sections, morphometric analysis, estimation of proinflammatory cytokines (IL-1p, IL-6, VEGF, TNFa and iNOS) expression were used to evaluate inflammatory response in myocardium. In addition, we estimated cardiac myosin antibody levels in blood serum and nitrite and nitrate levels in blood serum. Our data showed that immunization with cardiac myosin combined with IFA led to inflammatory response in the rat myocardium. Acute inflammation (i.e. lymphocyte infiltration of myocardium and increase of proinflammatory cytokines level) in M + IFA group occurred on 21 days after immunization.