Immunocorrection of apoptogenic effect of pertussis preparations with neutrophilokines
Tiukavkina, S.Iu.; Savis'ko, A.A.; Kharseeva, G.G.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2012(5): 60-63
2012
ISSN/ISBN: 0372-9311 PMID: 23163038 Document Number: 656526
Study the possibility of resistance increase of macrophages of experimental animals to apoptogenic effect of pertussis preparations with neutrophilokines. Apoptosis was evaluated in Coulter flow cytofluorimeter after staining with propidium iodide, as well as by characteristic morphologic changes in cells stained by histological preparations and by DNA fragmentation. The studies performed showed that pertussis preparations cause apoptosis of peritoneal macrophages leading to their alteration. Neutrophilokines induced by pertussis preparations were also established to suppress macrophage apoptosis. The results of our studies indicate the possibility to use neutrophilokines for immunocorrection of macrophage apoptosis induced by pertussis preparations.