Fast determination of phenolic acids in Lycium ruthenicum murr juice by solid phase extraction and HPLC
Chen, C.; Wen, H.; Zhao, X.; Tao, Y.; Shao, Y.; Mei, L.
Zhongguo Zhong Yao Za Zhi 36(7): 896-898
2011
ISSN/ISBN: 1001-5302 PMID: 21761730 Document Number: 653595
To establish a quantitative method for determination of gallic acid, original catechins, catechins, chlorogenic acid, caffeic acid, syringic acid in Lycium ruthenicum. The sample was separated on an ODS column (4. 6 mm x 250 mm, 5 pnm), eluted with methanol and water containing 0. 1% acid with detected wavelength at 280 nm, and flow rate at 1. 0 mL x min(-1). The linearity of six components were good (r = 0.9999). The average recoveries (n=5) of the six constituents were 97. 70% (RSD 2.3%), 99.64% (RSD 1.8%), 100.7% (RSD 2.1%), 99.98% (RSD 2.6%), 99.60% (RSD 2.2%), 99.04% (RSD 2.4%), respectively. The method is rapid and precise. It can be used for quality control of Lycium ruthenicum juice.