Enterovirus 71 induces apoptosis in a Bax dependent manner
Sun, Z.-m.; Xiao, Y.; Ren, L.-l.; Lei, X.-b.; Wang, J.-w.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 25(1): 49-52
2011
ISSN/ISBN: 1003-9279 PMID: 21789855 Document Number: 652538
To explore the molecular mechanism of apoptosis induced by enterovirus 71 (EV71) infection. The effects of EV71 on Rhabdomyosarcoma (RD) cell viability were detected by CCK8 assay. EV71-induced apoptosis on RD cells were detected by Hoechst 33342 staining and Western blot targeting Caspase 3, 8 and PARP. Bax conformational change was detected by immunoprecipitation with Bax 6A7 antibody. EV71 decreased the viability of RD cells and induces the activation of Caspase 3, 8 and PARP. Bax expression increases in RD cells after EV71 infection, and Bax conformational change also can be detected after EV71 infection. Our study reveals that EV71 induces Caspase-dependent apoptosis by Bax conformational change.