Omeprazole induces apoptosis in jurkat cells
Scaringi, L.; Cornacchione, P.; Ayroldi, E.; Corazzi, L.; Capodicasa, E.; Rossi, R.; Marconi, P.
International Journal of Immunopathology and Pharmacology 17(3): 331-342
2004
ISSN/ISBN: 0394-6320 PMID: 15461867 Document Number: 573914
We report for the first time a potent apoptotic effect of omeprazole (OM). Apoptosis was induced in Jurkat cells in a time and concentration-dependent mode. Caspase 3 and PARP were rapidly cleaved in response to OM, but apoptosis was only partially inhibited by the caspase 3 inhibitor DEVD-CHO. OM also induced an early lysosomal destabilization which increased progressively and was correlated with a parallel increase in apoptotic cells. The cysteine protease inhibitor E64d gave strong protection against apoptosis thus proving the involvement of lysosomal enzymes in OM-induced apoptosis whereas, it did not impede the caspase 3 cleavage. Instead ZVAD-fmk, a general caspase inhibitor, also able to inhibit cathepsin activity, protected cells completely from OM-induced apoptosis. It therefore seems that both caspases and cysteine cathepsins are involved in the execution stage of OM-induced apoptosis.