Effects of 2,2',4,4'-tetrabrominated diphenyl ethers on the TRalpha1 and TRbeta1 expression in liver in C57BL/6 mice
Liu, Z.; Liu, J.; Zhang, J.; Tian, Y.; Fang, D.; Huang, H.; Jiang, Y.; Zhou, J.; Zhang, H.; Mei, S.
Wei Sheng Yan Jiu 39(4): 399-403
2010
ISSN/ISBN: 1000-8020 PMID: 20726222 Document Number: 645923
To study the effect of 2,2',4,4'-tetrabrominated diphenyl ethers (BDE-47) on the expression change of TRalpha1, TRbeta1 at both mRNA and protein levels. C57BL/6 mice were divided randomly into 4 groups and administrated with corn oil, 0.5, 5 and 50 mg/kg/BW BDE-47 for 4 days through intraperitoneal injection respectively. GAPDH selected as internal standard, real time quantitative RT-PCR and Western blot were employed to detect the mRNA levels and protein of TRalpha1 and TRbeta1 in livers. Compared with the control group, the mRNA levels of TRalpha1 and TRbeta1 were up-regulated in the medium and high BDE-47 dose groups (P < 0.05). The protein level of TRalpha1 significantly up-regulated while TRbeta1 was significantly down-regulated. BDE-47 could change the expression of TRalpha1 and TRbeta1 in the mRNA and protein levels in liver.