Immunosuppressant dexamethasone can significantly extend the expression of hepatitis B virus antigens in the HBV mouse model by hydrodynamic transfection method
Guo, Y-Ju.; Wang, W.; Sun, S-Hui.; Zeng, D-Bing.; Zhao, G-Yu.; Yu, H.; Guo, Y.; Tan, W-Jie.; Lu, S-Chun.; Zhou, Y-Sen.
Bing du Xue Bao 26(1): 20-26
2010
ISSN/ISBN: 1000-8721 PMID: 20329554 Document Number: 644206
To develop a Hepatitis B virus (HBV) infection mouse model by hydrodynamic-based transfection and further to optimize the method of development of HBV infection mouse model, we first developed a construct which contained inverted terminal repeat elements (ITR) of adeno-associated virus (AAV) and 1.3 copies of HBV genome (ayw subtype). The pAAV-HBV1.3 DNA was then injected hydrodynamically into the tail veins of C57BL/6 mice in 5 seconds.