New original in vitro method to assess cholinesterase reactivity in organophosphate poisoning
Sorodoc, L.; Lionte, C.ăt.ăl.; Largu, E.; Petriş, O.
Revista Medico-Chirurgicala a Societatii de Medici Si Naturalisti Din Iasi 114(3): 757-763
2010
ISSN/ISBN: 0048-7848 PMID: 21235118 Document Number: 643721
Assessment of organophosphate poisoning could benefit from a safe, non-expensive, easy to perform, quick (< 1 hour) test, which evaluates the level of cholinesterase activity "in vitro" regarding to the capability of oximes to reactivate OF-blocked cholinesterase. In the proposed protocol, 0.5 mL of sample serum is incubated, prior to the evaluation of level of cholinesterase activity, with 5 microL of a Toxogonin dilution (0.125 mg) for 30 minutes at 37 degrees C. For the standardization of the newly proposed protocol, several important issues were documented in the present article. The new original method of assessing cholinesterase reactivability will consist in an advantage for the diagnosis, prognostic evaluation and therapeutic orientation in OF intoxication.