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Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin i

Shen, H.-H.; Hou, J.; Hu, Y.; Bai, B.-K.; Wang, Z.-J.; Yu, X.-H.; Kong, W.; Liu, Z.; Mao, P.-Y.

Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 24(1): 59-61

2010

ISSN/ISBN: 1003-9279
PMID: 20848854
Document Number: 642057
To develop a vector inserted with complete genome of poliovirus strain Sabin I. The 3 fragments of the complete genome of Sabin I was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified. The complete genome of poliovirus strain Sabin I was successfully cloned into vector pWSK29 with 9 nucleotide mutations. The complete genome plasmid was constructed and it provided a basis for further research of the function of Sabin I.

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