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Genome organization of the plasmid of IncQ/P4 group and its vector derivatives

Tsygankov, I.D.; Chistoserdov, A.I.

Genetika 22(11): 2606-2619

1986

ISSN/ISBN: 0016-6758
PMID: 3542706
Document Number: 280770
The genome organization and functioning of IncQ/P4 plasmids are reviewed. Based on these plasmids, cloning vectors have been constructed for broad host range of gram-negative bacteria. Together with one- and two-replicon vectors for cloning via insertion inactivation of markers, specialized plasmid vectors are described: cosmids, promoter-probe vectors, vectors for direct selection of recombinant molecules. Examples of using broad host range vectors for gene cloning and expression in non-enteric gram-negative bacteria are presented.

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