Plasmid RP4 as a vector replicor in genetic engineering
Jacob, A.E.; Grinter, N.J.
Nature 255(5508): 504-506
1975
ISSN/ISBN: 0028-0836 PMID: 1055860 Document Number: 84357
There is now considerable interest in the construction of plasmids containing Dna fragments from a variety of bacterial genera, for purposes of studying the expression of the genetic potentialities of the incorporated bacterial Dna in different bacterial hosts. We report that the self-transmissible plasmid RP4 has only one site susceptible to cutting by the enzyme Eco RI, and should therefore be a very suitable bacterial Dna vector in such experiments.
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