Simultaneous determination of multicomponent in Xuefuzhuyu granule with different UV-wavelength by HPLC
Liu, Z.; Ye, T.; Zhao, L.; Cheng, Y.; Luo, G.
Zhongguo Zhong Yao Za Zhi 35(16): 2157-2160
2010
ISSN/ISBN: 1001-5302 PMID: 21046752 Document Number: 639186
To establish the method for the quality control of Xuefuzhuyu granule. The content of Semen Armeniacae Amarum PE, Hydroxysafflor yellow A, paeoniflorin and ferulic acid in Xuefuzhuyu granule were determined by HPLC under multi-wavelength of 210, 403, 230, 316 nm. The calibration curve of Semen Armeniacae Amarum PE. hydroxysafflor yellow A, paeoniflorin and ferulic acid were linear within 0.1154-0.9232 (r = 0.9998), 0.0219-0.1754 (r = 0.9994), 0.3952-3.1616 (r = 0.9995), and 0.0426-0.3408 microg (r = 0.9998), respectively. The average recoveries were 101.78% (RSD was 1.7%), 99.60% (RSD was 2.9%), 98.90% (RSD was 2.0%), and 100.31% (RSD was 1.8%), respectively. The method of quantification is accurate, rapid and reliable for the quality control of Xuefuzhuyu granule.