Simultaneous determination of 4 major components in semen cassiae obtusifoline by HPLC
Su, H.; Wang, Z.; Tang, L.
Zhongguo Zhong Yao Za Zhi 36(10): 1327-1329
2011
ISSN/ISBN: 1001-5302 PMID: 21837976 Document Number: 648965
To establish a HPLC method for the simultaneous determination of 4 major components in semen cassiae obtusifoline and provide valuable data for quality control of semen cassiae obtusifoline. Kromasil 100-5 C18 column (4.6 mm x 250 mm, 5 microm). Eluted with water: acetonitrile: tetramethylene oxide: glacial acetic acid (100: 23: 5:1). Detecte at 278 nm. The flow rate was 1.0 mL x min(-1). Four compositions were separated well. Good linearies were obtained within the range of 0.274-1.37 microg, 0.153-0.765 microg, 0.302-1.51 microg and 0.052-0.26 microg for rubrofusarin gentiobioside, casside, aurantio-obtusin-6-O-beta-D- glucopyranoside, casside B respectively. The average recoveries were 100.9% (RSD 1.8%),101.2% (RSD 1.2%), 99.40% (RSD 2.2%), 100.5% (RSD 1.6%). The method is accurate, reliable and convenient. It can be used to control the quality of semen cassiae obtusifoline and its products.