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Application of 293 cells in in vitro detection of carcinogens

Zhao, R.; Zhang, L.; Zhong, R.-G.; Zhou, L.; Zeng, Y.

Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 23(1): 47-49

2009

ISSN/ISBN: 1003-9279
PMID: 19799017
Document Number: 637868
To investigate the application of 293 cells to detect suspected carcinogens and provide experimental evidence by using in vitro cell transformation assay and tumorigenicity study. The transformation systems of cells cultured in vitro have been adopted to clarify the tumor promotive activity of Microcystin LR (MC-LR). The malignant transformation of 293 cells induced by MC-LR is tested by several methods including clone forming in soft agarose, serum requirement assay and tumor forming in mice to define the promotive activity of 293 cells. 293 cell acted like tumor cells after induced by MC-LR: serum dependence decreased, anchorage independence growth in soft agarose and formed cell clones, malignant tumors appeared in SCID mice. 293 cells were easy to culture and sensitive to environmental carcinogens so that can be used in detection of suspicious carcinogens.

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