Rapid technique for the detection of carcinogens using mice liver microsomes
Jagota, S.K.
Indian Journal of Medical Research 94: 298-300
1991
ISSN/ISBN: 0019-5340 PMID: 1720422 Document Number: 386190
Mice liver microsomes were prepared at low g (10,000 g) force by Ca(2+)-aggregation method and were used for the detection of carcinogens by degranulation technique. RNA/protein ratio of these microsomes was 0.177 which was comparable to rat liver microsomes. Per cent degranulation with two carcinogens (O-dianisidine and benzidine) at 20 micrograms concentration was 21 per cent on the basis of RNA/protein ratio basis with both the carcinogens. At 40 micrograms concentration the per cent degranulation was observed to be almost double. Both the carcinogens required NADPH for their activation.