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An indirect radiolabelled antibody staining technique for the rapid detection and identification of bacteria

Benbough, J.E.; Martin, K.L.

Journal of Applied Bacteriology 41(1): 47-58

1976

ISSN/ISBN: 0021-8847
PMID: 821910
Document Number: 106473
Small numbers of Bacillus subtilis var. niger spores, Serratia marcescens and Francisella tularensis can be detected and identified in 1 h using an indirect radiolabeled antibody staining technique with homologous rabbit antibacterial antibody and radiolabeled goat anti-rabbit globulin. 3H is better than 125I, 14C and 35S for labeling the globulin. The reagent obtained is sensitive and adequately stable.

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