Determination of ellagic acid compounds in Euscaphis japonica by using UPLC
Wang, A.; Liu, Z.; Wang, H.; Li, Y.; Liao, S.; Wang, Y.
Zhongguo Zhong Yao Za Zhi 34(24): 3235-3238
2009
ISSN/ISBN: 1001-5302 PMID: 20353008 Document Number: 636692
To develop an ultra performance liquid chromatography (UPLC) method for simultaneous determination of five ellagic acids in Euscaphis japonica. Analysis was carried out on an Acquity BEH C18 (2.1 mm x 100 mm, 1.7 microm) column at 40 degrees C eluted with acetonitrile-0.1% phosphoric acid aqueous solution as mobile phases in gradient elution. The flow rate was 0.2 mL x min(-1), and the detection wavelength set was monitored at 245 nm. All calibration curves showed good linear relationship within test ranges (r >0.9997), and the overall recoveries were in the range of 97.2%-102.1%, with RSD less than 3.2% (n = 6). The overall RSD of precision test were less than 2.9%. The developed method was simple, rapid, accurate and reproducible, and can be used for the quality control of E. japonica.