Use of mathematical model for determination of virus neutralizing activity of antibodies during experimental SHIV-infection
Klots, I.N.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2: 68-70
2009
ISSN/ISBN: 0372-9311 PMID: 19459482 Document Number: 635132
To study the role of neutralizing antibodies (NA) on the model of SHIV-infection in pig-tailed macaques immunized by different methods. Titers of NA were determined using TZM-bl cells; titers corresponding to 50% neutralization were determined from serum dilution--relative luminescence graph. We have proposed to use index of integral neutralizing activity, which mathematically expressed as integral of neutralization function in serum dilutions interval from 1 (or first dilution used) to that giving selected titer (we selected 50% neutralization). On the basis of analysis of data on course of SHIV-infection in previously immunized macaques it was concluded that more clear correlation exists between results of infection and integral neutralizing activity of serum compared with NA titers corresponding to 50% neutralization. Proposed method could be useful for analysis of immunization efficacy against HIV infection.