Effect of rat recombinant leptin on expression of SOCS-3 in mature adipocytes
Liu, L.; Gu, H.; Yang, J.; Yu, F.
Wei Sheng Yan Jiu 38(2): 160-162
2009
ISSN/ISBN: 1000-8020 PMID: 19408656 Document Number: 634990
To study the effect of rat recombinant leptin on expressions of suppressor of cytokine signaling 3 (SOCS-3) mRNA and protein in mature adipocytes . Mature adipocytes were separated from epididymal adipose tissues of male SD rats, and the expressions of long-form leptin receptor mRNA were detected. The separated adipocytes were then treated with rat recombinant leptin at the doses of 0.5-500 nmol/L for 0.5 to 6h. The expressions of SOCS-3 mRNA were measured with RT-PCR and proteins were measured with immuniprecipitation combined with Western blot analysis. Low-level expressions of long-form leptin receptor mRNA could be measured in mature adipocytes. The adipocytes expressed small amounts of SOCS-3 mRNA and protein witbout leptin treatment, and no significant changes were observed at 0.5 Snmol/L of leptin. However, tbe expressions of SOCS-3 mRNA and protein increased significantly at the doses of 5-500 nmol/L of leptin in the consistent with treatment time. Leptin could have direct effects on adipocytes. High leptin concentration could induce the expressions of SOCS-3 mRNA and protein in mature adipocytes.