Adrenergic regulation of the uncoupling protein expression in foetal rat brown adipocytes in primary culture
Porras, A.; Fernández, M.; Benito, M.
Biochemical and Biophysical Research Communications 163(1): 541-547
1989
ISSN/ISBN: 0006-291X PMID: 2549996 Document Number: 338380
The adrenergic and T3 modulation of UCP expression in non-proliferative foetal brown adipocyte primary cultures were studied. The UCP in the cultured cells was determined by immunological detection of the protein and by quantification of the mitochondrial GDP-binding. Our results showed a relative increase of 65-75% in UCP levels and 60-80% in the mitochondrial GDP-binding capacity under .beta.-adrenergic stimulatory conditions, while neither .alpha.1-adrenergic agonists nor T3 showed an effect.