Two CpG-islands of SEMA3B gene: methylation in clear cell renal cell carcinoma
Loginov, V.I.; Khodyrev, D.S.; Pronina, I.V.; Maliukova, A.V.; Kazubskaia, T.P.; Ermilova, V.D.; Gar'kavtseva, R.F.; Zabarovskiĭ, E.R.; Braga, E.A.
Molekuliarnaia Biologiia 43(6): 1088-1092
2009
ISSN/ISBN: 0026-8984 PMID: 20088387 Document Number: 634865
Earlier in some small cell lung (SCLC) and non-small cell lung carcinoma (NSCLC) cell lines, methylation of CpG-island was found in the SEMA3B region, which belongs to the first intron according to the NCBI data base (Build 36). The aim of this work was to study methylation of two SEMA3B CpG-islands: promoter and intronic in clear cell renal cell carcinoma (RCC). Using methyl specific PCR and bisulfite sequencing, it was shown for the first time that promoter CpG-island was methylated in RCC with high frequency 56% (34/61), and intronic CpG-island - with somewhat lower frequency 35% (17/48). Significant reverse correlation was estimated between mRNA level decrease and methylation of promoter CpG-island in RCC for the first time (P < or = 0.05 by Fisher's exact test), no correlation was determined for intronic CpG-island. This result suggested that methylation of promoter CpG-island contributed into inactivation of SEMA3B gene-suppressor in RCC.